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Research Article 


Keratin-Associated Protein (Kap) 8 Gene Polymorphism And Its Association With Wool Traits In Rambouillet Sheep

Vikas Mahajan*1, A K Das2, R K Taggar3, Dhirendra Kumar4 And Nazam Khan5.

Abstract
In the present study, KAP 8 gene polymorphism and its association with wool traits in Rambouillet sheep was investigated by using SSCP-PCR. Genomic DNA was isolated from blood samples of 100 Rambouillet sheep. A 194 bp segment was amplified by PCR using ovine specific primers for KAP 8 gene. The results identified 3 genotypes, viz. AA, AB and BB with genotype frequencies were 0.14 (14), 0.44 (44) and 0.42 (42) respectively with allele frequencies for allele A (0.36) and allele B (0.64). The χ-test showed that the genotype distribution was in agreement with Hardy-Weinberg equilibrium (HWE). KAP 8 gene has significantly higher greasy fleece weight (P<0.01) in males whereas females have significantly (P<0.01) superior fibre diameter, higher staple length and wool count. AA genotype has significantly (P<0.05) superior fibre diameter followed by BB and AB genotype. AB and AA genotypes have significantly (P<0.01) higher greasy fleece weight followed by BB genotype. From the study, it may be concluded that KAP 8 gene might be a potential molecular marker for genetic selection for superior fibre diameter and greasy fleece weight in Rambouillet sheep.

Key words: Keratin Associated Protein 8, PCR-SSCPP, Polymorphisms, Rambouillet Sheep, Wool Traits


 
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How to Cite this Article
Pubmed Style

Vikas Mahajan, A K Das, R K Taggar, Dhirendra Kumar And Nazam Khan. Keratin-Associated Protein (Kap) 8 Gene Polymorphism And Its Association With Wool Traits In Rambouillet Sheep. IJLSAS. 2019; 1(1): 50-57.


Web Style

Vikas Mahajan, A K Das, R K Taggar, Dhirendra Kumar And Nazam Khan. Keratin-Associated Protein (Kap) 8 Gene Polymorphism And Its Association With Wool Traits In Rambouillet Sheep. http://www.ijlsas.com/?mno=302644888 [Access: January 30, 2020].


AMA (American Medical Association) Style

Vikas Mahajan, A K Das, R K Taggar, Dhirendra Kumar And Nazam Khan. Keratin-Associated Protein (Kap) 8 Gene Polymorphism And Its Association With Wool Traits In Rambouillet Sheep. IJLSAS. 2019; 1(1): 50-57.



Vancouver/ICMJE Style

Vikas Mahajan, A K Das, R K Taggar, Dhirendra Kumar And Nazam Khan. Keratin-Associated Protein (Kap) 8 Gene Polymorphism And Its Association With Wool Traits In Rambouillet Sheep. IJLSAS. (2019), [cited January 30, 2020]; 1(1): 50-57.



Harvard Style

Vikas Mahajan, A K Das, R K Taggar, Dhirendra Kumar And Nazam Khan (2019) Keratin-Associated Protein (Kap) 8 Gene Polymorphism And Its Association With Wool Traits In Rambouillet Sheep. IJLSAS, 1 (1), 50-57.



Turabian Style

Vikas Mahajan, A K Das, R K Taggar, Dhirendra Kumar And Nazam Khan. 2019. Keratin-Associated Protein (Kap) 8 Gene Polymorphism And Its Association With Wool Traits In Rambouillet Sheep. International Journal Of Life Sciences and Applied Sciences, 1 (1), 50-57.



Chicago Style

Vikas Mahajan, A K Das, R K Taggar, Dhirendra Kumar And Nazam Khan. "Keratin-Associated Protein (Kap) 8 Gene Polymorphism And Its Association With Wool Traits In Rambouillet Sheep." International Journal Of Life Sciences and Applied Sciences 1 (2019), 50-57.



MLA (The Modern Language Association) Style

Vikas Mahajan, A K Das, R K Taggar, Dhirendra Kumar And Nazam Khan. "Keratin-Associated Protein (Kap) 8 Gene Polymorphism And Its Association With Wool Traits In Rambouillet Sheep." International Journal Of Life Sciences and Applied Sciences 1.1 (2019), 50-57. Print.



APA (American Psychological Association) Style

Vikas Mahajan, A K Das, R K Taggar, Dhirendra Kumar And Nazam Khan (2019) Keratin-Associated Protein (Kap) 8 Gene Polymorphism And Its Association With Wool Traits In Rambouillet Sheep. International Journal Of Life Sciences and Applied Sciences, 1 (1), 50-57.